Test Method
The B&F test is performed by inoculating a sterile test medium, typically Trypticase Soy Broth (TSB) or Fluid Thioglycollate Medium (FTM), with representative test microorganisms. This medium is then brought into contact with the test product and incubated for 14 days at the prescribed temperatures:

• For bacteria: 30 °C – 35 °C
• For fungi and yeasts: 20 °C – 25 °C

During incubation, careful monitoring is conducted to verify whether the test organisms can multiply in the presence of the product. Successful microbial growth confirms that the test product does not have bacteriostatic or fungistatic properties that could influence sterility test results.

Thus, the B&F test serves as a crucial quality control step in sterility testing, contributing to the reliability of final test results.